Thermo Fisher Scientific Cyclin B1 Polyclonal Antibody
Cyclin B1 단백질을 인식하는 Rabbit Polyclonal Antibody로 인간 시료에 반응합니다. Western blot 및 Immunocytochemistry에 적합하며, 고순도의 항원 친화 크로마토그래피로 정제되었습니다. 세포주기 G2/M 조절 연구에 활용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 0.04–0.4 µg/mL
Immunocytochemistry (ICC/IF)
- Tested Dilution: 0.25–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human CCNB1. Recombinant protein control fragment (Product #RP-104614) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2790309 |
Product Specific Information
Immunogen sequence:
PVPVSEPVPE PEPEPEPEPV KEEKLSPEPI LVDTASPSPM ETSGCAPAEE DLCQAFSDVI LAVNDVDAED GAD
Target Information
Cyclins bind to and regulate the activity of Cyclin-dependent protein kinases (CDKs). Cyclin B1 (CCNB1) is a regulatory protein essential for controlling the cell cycle at the G2/M transition. It forms a complex with p34 (cdc2) to create the maturation-promoting factor (MPF).
Two alternative transcripts exist: one constitutively expressed and another cell cycle-regulated, predominantly during G2/M phase. Cyclin B1 accumulates during G2 phase and is degraded at the end of mitosis via APC-dependent destruction.
Overexpression of Cyclin B1 has been observed in various cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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