
Thermo Fisher Scientific Eosinophil Peroxidase (EPX) Monoclonal Antibody (AHE-1)
인간 Eosinophil Peroxidase(EPX)를 인식하는 단클론 항체로, 면역세포 내 과립 단백질 특이적 검출에 적합합니다. ICC/IF, Flow Cytometry, ELISA에 사용 가능하며, MCF-7 세포 및 편도 조직에서 양성 대조로 활용됩니다. 단백질 A/G로 정제된 액상 항체로 4°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 2–4 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/mL |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AHE-1 |
| Immunogen | Human eosinophils from a patient with hypereosinophilic syndrome |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Stability: Antibody is stable for 24 months.
- Positive Control: MCF-7 cells, Tonsil
- Cellular Localization: Cytoplasmic, granular
Specificity Comments:
Peripheral blood granulocytes are classified into neutrophils, basophils, and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiological or pharmacological stimuli and play important roles in immune responses.
Eosinophil major basic protein and eosinophil peroxidase (EPX) are specific to eosinophils. The AHE-1 clone recognizes human EPX and does not cross-react with other granule proteins such as eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin.
This monoclonal antibody stains only eosinophils and does not stain other peripheral blood cells (platelets, neutrophils, monocytes, lymphocytes, or red blood cells).
Human EPX can form a tetramer consisting of two light and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).
Target Information
EPX belongs to the peroxidase family, XPO subfamily.
Defects in EPX are associated with eosinophil peroxidase deficiency (EPD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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