Thermo Fisher Scientific ACBD3 Recombinant Rabbit Monoclonal Antibody (23GB3620)
Rabbit monoclonal antibody against human ACBD3, validated for WB, ICC/IF, and Flow Cytometry. Recombinant antibody produced in HEK293F cells. High specificity and reproducibility for research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| Flow Cytometry (Flow) | 1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 23GB3620 |
| Immunogen | A synthetic peptide of human ACBD3 (1–61 AA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Wet ice (domestic); Dry ice (international) |
Target Information
GOLPH1, also known as GCP60, is a Golgi protein that interacts with the integral membrane protein giantin and contributes to the maintenance of Golgi structure. It also interacts with Golgi proteins such as Golgin-160, which can be cleaved by caspases-2, -3, and -7, leading to nuclear localization of Golgin-160. The interaction between GOLPH1 and Golgin-160 fragments is regulated by the oxidation state of Cys-463, suggesting a coordinated mechanism for nuclear localization. Additionally, GOLPH1 interacts with Numb, a cytosolic signaling protein involved in asymmetric cell division, indicating that Golgi dynamics may regulate Numb signaling and link cell fate with cell cycle progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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