Thermo Fisher Scientific SARS Coronavirus Spike Protein Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 600-700 of coronavirus Spike (NP_8288511)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.04 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914333

Product Specific Information

Positive test controls include: 293T.

Immunogen sequence: DVNCTDVSTA IHADQLTPAW RIYSTGNNVF QTQAGCLIGA EHVDTSYECD IPIGAGICAS YHTVSLLRST SQKSIVAYTM SLGADSSIAY SNNTIAIPTN F

Target Information

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosylated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.