Thermo Fisher Scientific PGP9.5 Polyclonal Antibody
PGP9.5 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, 신경세포 특이적 마커 연구에 적합합니다. Western blot, IHC, ICC/IF 등 다양한 응용 가능하며, 고순도 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:20,000 | - |
| Immunohistochemistry (IHC) | - | View 6 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 137–223 of Human PGP9.5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.13 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2546488 |
Product Specific Information
- Recommended positive controls: 293T, A549, H1299, Neuro2A, GL261, rat brain, PC-12, mouse brain
- Store product as a concentrated solution
- Centrifuge briefly prior to opening the vial
Target Information
PGP9.5 (Protein Gene Product 9.5, also known as UCH-L1 or PARK5) is a neuron-specific protein distinct from neuron-specific enolase, with a molecular weight of approximately 27 kDa. It belongs to the ubiquitin carboxyl-terminal hydrolase family (peptidase family C12) and possesses both hydrolase and ligase activities that generate ubiquitin monomers.
PGP9.5 is expressed in neurons and nerve fibers throughout the central and peripheral nervous systems, neuroendocrine cells, renal tubules, spermatogonia, Leydig cells, and ovarian tissues. Overexpression is associated with non-small cell lung cancer, while reduced expression correlates with Huntington and Alzheimer diseases. It serves as a useful neuronal marker in studies of neurodegenerative disorders such as Parkinson’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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