Thermo Fisher Scientific Proprotein Convertase 2 Polyclonal Antibody
Human, Mouse, Rat에 반응하는 Rabbit Polyclonal Antibody로 Western blot, IHC, ICC, ELISA 등에 사용 가능. Proprotein Convertase 2의 201-300 aa 유래 펩타이드로 제작됨. Protein A로 정제된 액상 항체로 -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human Proprotein Convertase 2 (amino acids 201–300) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The subtilisin-like prohormone convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins transported by the constitutive secretory pathway are also targeted for maturation by PCs.
PC family members share structural similarities, including:
- A heterogeneous ~10 kDa amino-terminal proregion
- A highly conserved ~55 kDa subtilisin-like catalytic domain
- A carboxyl-terminal domain heterogeneous in length and sequence
These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment.
Immunolocalization studies show that PC1 is found in the perinuclear region and trans-Golgi network, whereas PC2 is found in the trans-Golgi network and diffusely distributed in the peripheral cytoplasm.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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