Thermo Fisher Scientific WWTR1 Monoclonal Antibody (OTI1B5), TrueMAB
인간 WWTR1 단백질을 인식하는 단클론 항체로 Western blot, ICC/IF, Flow cytometry에 적합. IgG1 아이소타입, 무보존제, 동결건조 형태. Hippo 신호 경로 관련 연구에 활용 가능.
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- CF503396
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Thermo Fisher Scientific WWTR1 Monoclonal Antibody (OTI1B5), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B5 |
| Immunogen | Full length human recombinant WWTR1 protein produced in HEK293 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
WW domain-containing transcription regulator protein 1 (WWTR1) is a transcriptional coactivator acting downstream in the Hippo signaling pathway, which regulates organ size and tumor suppression by controlling cell proliferation and apoptosis.
This pathway involves a kinase cascade where STK3/MST2 and STK4/MST1, together with SAV1, activate LATS1/2 in complex with MOB1. MOB1 then phosphorylates and inactivates YAP1 and WWTR1/TAZ.
WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation, regulates SMADS nuclear accumulation, and couples them to transcriptional machinery such as the mediator complex. It also plays a role in stem-cell self-renewal, proliferation, and epithelial-mesenchymal transition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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