Thermo Fisher Scientific BTF Polyclonal Antibody
BCLAF1 단백질을 표적하는 Rabbit Polyclonal Antibody로 WB, IHC, IP 등에 사용 가능. 인간과 생쥐 반응성 보유. 항원 친화 크로마토그래피로 정제된 액상 형태. 4°C 보관, 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 1–10 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 150 and 200 of human BCL2-Associated Transcription Factor 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
BCLAF1 (BCL2-Associated Transcription Factor 1) is an intracellular, death-promoting transcriptional repressor that interacts with several members of the BCL2 family. It shows homology to the basic leucine zipper (bZIP) and MYB DNA binding domains.
In apoptotic cells, BCLAF1 localizes to dot-like structures throughout the nucleus and redistributes near the nuclear envelope. Overexpression induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. Also known as BTF, it triggers a proapoptotic signal and is mainly expressed in skeletal muscles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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