Thermo Fisher Scientific Glypican 1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Flow Cytometry (Flow)

1:50-1:200

-

ELISA (ELISA)

1:1,000-1:10,000

-

Immunoprecipitation (IP)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.71 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2545531

Product Specific Information

Recommended positive controls: A431, K562, MCF-7, AsPC-1, MDA-MB-231, MDA-MB-231 (24 µg/mL Tunicamycin for 16hr), U87-MG, U87-MG membrane extracts, U87-MG conditioned medium, Mouse brain, mouse fetal brain, A431 (500 units PNGase F for 4 hr), A431 (24 µg/mL Tunicamycin treatment for 16hr), A431 (4 mM Benzyl glucosinolate treatment for 32hr), A431(24 µg/mL Tunicamycin treatment for 16hr and 4 mM Benzyl glucosinolate treatment for 32hr).

Predicted reactivity: Mouse (89%), Rat (88%), Rhesus Monkey (97%), Bovine (89%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.