Shapton Medium
Details
Shapton Medium is recommended for enumeration of spores of Bacillus stearothermophilus which causes flat sour spoilage in canned food with pH more than 4.5. Tryptone, Peptone, HM peptone B and yeast extract in the medium provide carbon, nitrogen, vitamins and minerals required for bacterial metabolism. Dextrose is the fermentable carbohydrate. Bromocresol purple is the pH indicator, indicating dextrose fermentation visualized as a colour change from purple to yellow. Bacillus stearothermophilus grows luxuriantlly on this medium giving yellow colored colony.
