Thermo Fisher Scientific Phospho-A-Raf (Ser299) Polyclonal Antibody
A-Raf 단백질의 Ser299 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat 반응성. 고순도 액상 제품으로 연구용에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ARAF (Accession P10398), corresponding to amino acid residues around phosphorylated Ser299 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817264 |
Product Specific Information
This antibody detects endogenous levels of A-RAF only when phosphorylated at Ser299.
Target Information
Several serine/threonine protein kinases function as intermediates in signal transduction pathways, including ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity and is broadly expressed in nearly all cell lines tested. It is the cellular homolog of v-Raf, the transforming gene product of the 3611 strain of murine sarcoma virus.
A-Raf, a second member of the Raf gene family, shows substantial homology to Raf-1 within the kinase domain but less elsewhere. A-Raf expression is highest in urogenital tissues and kidney, and lowest in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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