Thermo Fisher Scientific Platinum Taq Monoclonal Antibody

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

-

Inhibition Assays (IA)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Class

Monoclonal

Type

Antibody

Immunogen

Taq DNA Polymerase

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

purified

Storage buffer

TBS, pH 8, with 2mM Na2PO4, 0.1mM EDTA, 50% glycerol, 1mM DTT

Contains

no preservative

Storage conditions

-20°C

RRID

AB_2532987

Product Specific Information

One unit of Taq DNA polymerase incorporates 10 nmol of deoxyribonucleotide into DNA in 30 minutes at 74ºC. One unit is defined as the amount of product required to inhibit one unit of Taq DNA polymerase.

PCR Functional Assay Specifications: PCR reactions containing 100 ng, 10 ng, and 5 ng of input human genomic DNA yield 40 ng, 10 ng, and 5 ng of an 800 bp target, respectively, when observed on an ethidium bromide stained gel.

In a radioactive nucleotide incorporation assay, Taq DNA polymerase activity has been successfully inhibited by 10965-010.

Target Information

Platinum® Taq Antibody is a proprietary inhibitor which, when bound to Taq DNA polymerase, inactivates polymerase activity. This reagent provides an automatic ″hot start″ for Taq DNA polymerase in PCR. Hot starts are typically used in PCR to increase sensitivity, specificity, and yield while allowing assembly of reactions at ambient temperatures. The extra time, effort, and contamination risks associated with manual hot start procedures are addressed with the use of Platinum® Taq Antibody.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.