Thermo Fisher Scientific EP300 Monoclonal Antibody (1B1)
Human reactive monoclonal antibody against EP300, clone 1B1. Validated for WB, IHC, ICC/IF, and ELISA. Affinity purified, unconjugated liquid form. Stable at -20°C for research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 3 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1B1 |
| Immunogen | EP300 (NP_001420, 731–830 a.a.) partial recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein:
PPLQHHGQLA QPGALNPPMG YGPRMQQPSN QGQFLPQTQF PSQGMNVTNI PLAPSSGQAP VSQAQMSSSS CPVNSPIMPP GSQGSHIHCP QLPQPALHQN
Target Information
EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein.
p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB.
This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A.
EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송 정보
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