Thermo Fisher Scientific Aldolase A/B/C Recombinant Rabbit Monoclonal Antibody (PSH01-81)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2000-1:5000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:20,000

-

Immunohistochemistry (Frozen) (IHC (F))

1:1,000

-

Immunocytochemistry (ICC/IF)

1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

PSH01-81

Immunogen

Synthetic peptide within human aldolase c aa 50-100. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Aldolase A/B/C,uniProtId:P09972-1,ncbiNodeId:9606,antigenRange:50-100,antigenLength:364,antigenImageFileName:MA5-56379_Aldolase_ABC_P09972-1_Rabbit.svg,antigenImageFileNamePDP:MA5-56379_Aldolase_ABC_P09972-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

Positive Control: U-87 MG cell lysate, RAW264.7 cell lysate, C6 cell lysate, human brain tissue lysate, mouse brain tissue lysate, mouse pancreas tissue lysate, rat brain tissue lysate, rat pancreas tissue lysate, U-87 MG, mouse brain tissue, rat brain tissue.

Tissue Specificity: Group enriched (brain, retina).

Subcellular Location: cytoskeleton, cytosol, extracellular exosome, extracellular region.

Sequence Similarities: 100% Mouse/Rat.

Predicted band size: 110 kDa.

Target Information

Aldolases are important glycolytic cytosolic enzymes which catalyse the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate. There are three aldolase isozymes coded by three distinct genes in mammals, namely aldolases A, B, and C. Aldolase A is heavily expressed in muscle enzyme and aldolase B is heavily expressed in liver and is regarded as a liver specific enzyme. In the adult, aldolase C is the brain-specific isozyme expressed in astrocytes and a few classes of neurons, notably Purkinje cells. Appropriate antibodies to aldolase C are therefore useful to identify astrocytes in cell culture and sectioned material, and the enzyme may be over expressed in some forms of cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.