Thermo Fisher Scientific CPSF4 Polyclonal Antibody

Applications

• Western Blot (WB): 1:500–1:3,000

Product Specifications

Product Specific Information

• Recommended positive controls: 293T, A431, Raji
• Predicted reactivity: Mouse (98%), Rat (97%), Chicken (93%), Rhesus Monkey (100%), Bovine (97%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4 (CPSF4), an essential component of the 3′ end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with CPSF4. Binding of the NS1 protein to CPSF4 prevents CPSF binding to the RNA substrate and inhibits 3′ end cleavage and polyadenylation of host pre-mRNAs. Thus, the NS1 protein selectively inhibits the nuclear export of cellular, but not viral, mRNAs. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.