Thermo Fisher Scientific Phospho-MYL9/MYL12 (Ser20) Polyclonal Antibody
Phospho-MYL9/MYL12 (Ser20) 폴리클로날 항체로 인간 및 생쥐 반응성 확인. Western blot, IHC, ELISA 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 고순도 제품. 근육 수축 조절 및 CD69-Myl9/12 상호작용 연구에 적합.
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Thermo Fisher Scientific Phospho-MYL9/MYL12 (Ser20) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 2–5 µg/mL |
| Immunohistochemistry (Frozen) (IHC-F) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 1:10,000–1:30,000 |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide corresponding to residues K(12) K R P Q R A T (pS) N V F A M F D(27) of the human Myosin Light Chain protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.19 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_795761 |
Product Specific Information
- Recommended positive controls: Cardiac myocytes
- PA1-26470 is expected to cross-react with all mammals and birds due to 100% sequence homology.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Myosin regulatory light polypeptide 9 is a highly conserved, ubiquitously expressed protein encoded by the MYL9 gene in humans.
Myosin, a structural component of muscle, consists of two heavy chains and four light chains, including both nonphosphorylatable and phosphorylatable subunits.
MYL9 may regulate muscle contraction by modulating the ATPase activity of myosin heads.
MYL9 and MYL12 are functional ligands for CD69; blockade of CD69–Myl9/12 interaction ameliorates allergic airway inflammation in mouse models of asthma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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