Thermo Fisher Scientific DBI Polyclonal Antibody
Human, Mouse, Rat 반응성을 가진 Rabbit Polyclonal Antibody로 DBI 단백질 검출에 적합. WB, IHC, ICC, ELISA, IP 등 다양한 응용 가능. 고순도 Affinity Chromatography 정제, 안정적 액상 형태로 -20°C 보관.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–104 of human DBI (NP_001171513.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.84 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805381 |
Product Specific Information
Immunogen sequence:
MWGDLWLLPP ASANPGTGTE AEFEKAAEEV RHLKTKPSDE EMLFIYGHYK QATVGDINTE RPGMLDFTGK AKWDAWNELK GTSKEDAMKA YINKVEELKK KYGI
Positive Samples: HT-29, THP-1, MCF-7, NCI-H460, A673, U-937
Cellular Location: Endoplasmic reticulum, Golgi apparatus
Target Information
The DBI gene encodes diazepam binding inhibitor, a protein regulated by hormones and involved in lipid metabolism and displacement of beta-carbolines and benzodiazepines. It modulates signal transduction at type A GABA receptors in brain synapses. The protein is conserved from yeast to mammals, with a highly conserved hydrophobic binding site for medium- and long-chain acyl-CoA esters. DBI also mediates feedback regulation of pancreatic secretion and postprandial release of cholecystokinin, and acts as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes are located on chromosomes 6, 8, and 16. Multiple transcript variants encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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