
Takara BstPI (BstEII, EcoO65I) restriction enzyme
Thermophilic restriction enzyme BstPI (BstEII, EcoO65I), recognizing GGTNACC sites. Supplied with buffer H, optimal at 60°C. Activity affected by CG methylation. High-quality tested for overdigestion, ligation, and cloning performance.
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BstPI (BstEII, EcoO65I) Restriction Enzyme
Recognition Site
G | GTNAC C
C CANTG | GProduct Information
- Supplied buffer: H
- Effect of DNA methylation: Enzyme activity is affected by CG methylase
- Optimal reaction temperature: 60°C
Specifications
| 항목 | 내용 |
|---|---|
| Source | Bacillus stearothermophilus |
| Concentration | 10 units/μl |
| Substrate for unit definition | Lambda DNA |
| Star activity | Unrelated sites may be cut at high glycerol concentration and low ionic strength |
| Quality testing | Overdigestion, genome DNA analysis, ligation-recutting, and pKF3 cloning |
Additional Product Information
Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
Kit Components Lists and Certificates of Analysis are available under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566
Asia Pacific: +1.650.919.7300
Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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