Thermo Fisher Scientific SHH Polyclonal Antibody
SHH 단백질을 인식하는 염소 다클론 항체로, WB, ICC/IF, Flow Cytometry에 사용 가능. 인간 및 생쥐 반응성. 액상 형태로 보관하며 TBS 버퍼 포함. 배아 발생 관련 Sonic Hedgehog 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–3 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 10 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide with sequence C-DSEALHPLGMAVK (aa 448–460) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2932848 |
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:32,000.
Target Information
Sonic Hedgehog (SHH) is expressed during embryogenesis and plays a critical role in early embryo patterning, including ventral neural tube and limb axis formation. The SHH precursor undergoes autocatalytic cleavage, producing an N-terminal signaling fragment and a C-terminal processing fragment. The C-terminal fragment attaches a cholesterol group to the N-terminal product, limiting its diffusion. Defects in SHH or its pathway can cause holoprosencephaly (HPE) and may be linked to VACTERL syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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