Thermo Fisher Scientific SP1 Polyclonal Antibody
SP1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF 등에 사용 가능. Human, Mouse, Rat 시료에 반응하며, PBS+50% glycerol buffer에 보관. Affinity chromatography로 정제되어 높은 특이성과 재현성을 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human SP1 (Accession P08047), corresponding to amino acid residues T427–P477 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2900588 |
Product Specific Information
Antibody detects endogenous levels of total SP1.
Target Information
SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. It binds with high affinity to GC-rich motifs and regulates the expression of numerous genes involved in processes such as cell growth, apoptosis, differentiation, and immune responses. SP1 activity is highly regulated by post-translational modifications including phosphorylation, sumoylation, proteolytic cleavage, glycosylation, and acetylation. It also binds the PDGFR-alpha G-box promoter and may modulate cellular responses to DNA damage. SP1 is implicated in chromatin remodeling and plays a role in recruiting SMARCA4/BRG1 to the c-FOS promoter, as well as in regulating FE65 gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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