
Thermo Fisher Scientific Rabbit anti-Goat IgG Fc Cross-Adsorbed Secondary Antibody, HRP
Thermo Fisher Scientific의 Rabbit anti-Goat IgG Fc HRP 결합 2차 항체로, WB, IHC, ELISA 등에 사용 가능. Goat IgG Fc에 특이적이며 인간 혈청 단백질에 교차 반응 없음. 고감도 검출용 HRP 결합 폴리클로날 항체로 신뢰성 높은 면역분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobin Fc region |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2534819 |
| Target | IgG Fc |
| Cross Adsorption | Against human serum proteins |
| Antibody Form | Whole Antibody |
Additional Formats
- [AP Conjugate](/antibody/secondary/product-name/Goat IgG Fc Cross-Adsorbed/conjugates/AP/host/Rabbit/)
- [Biotin Conjugate](/antibody/secondary/product-name/Goat IgG Fc Cross-Adsorbed/conjugates/Biotin/host/Rabbit/)
- Request Custom Conjugation
Product Specific Information
Rehydrate with 1.1 mL of deionized water and let stand for 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove particulates. Prepare fresh working dilution daily. Store lyophilized material at 2–8°C. For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin goat serum immunoglobulins, light chains on all goat immunoglobulins, goat IgG F(ab')₂ fragment, or human serum proteins.
Target Information
Anti-Goat secondary antibodies are affinity-purified with well-characterized specificity for goat immunoglobulins. They are useful for detection, sorting, or purification of target proteins. Secondary antibodies enhance versatility, allowing use with various detection systems (HRP, AP, fluorescence) and provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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