Thermo Fisher Scientific ATP6V0A1 Polyclonal Antibody
ATP6V0A1 단백질을 인식하는 Rabbit Polyclonal 항체로, IHC, ICC/IF 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, Human 시료에 반응. PBS 기반 완충액에 보관되며 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 | - |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ATP6V0A1. Recombinant protein control fragment (Product #RP-93230). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2638371 |
Product Specific Information
Immunogen sequence:
VQFRDLNPDV NVFQRKFVNE VRRCEEMDRK LRFVEKEIRK ANIPIMDTGE NPEVPFPRDM IDLEANFEKI ENELKEINTN QEALKRNFLE LTELK
Highest antigen sequence identity to the following orthologs:
- Mouse: 100%
- Rat: 100%
Target Information
ATP6V0A1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles.
V-ATPase–dependent organelle acidification is essential for intracellular processes such as:
- Protein sorting
- Zymogen activation
- Receptor-mediated endocytosis
- Synaptic vesicle proton gradient generation
V-ATPase consists of:
- V1 domain (cytosolic): Three A and three B subunits, two G subunits, plus C, D, E, F, and H subunits (contains ATP catalytic site)
- V0 domain (transmembrane): Five subunits (a, c, c’, c”, and d)
Additional isoforms of many subunits are encoded by multiple genes or alternatively spliced variants.
The encoded ATP6V0A1 protein is associated with clathrin-coated vesicles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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