Thermo Fisher Scientific ABCA1 Polyclonal Antibody, DyLight 650
ABCA1 단백질을 인식하는 DyLight 650 결합 폴리클로날 항체로, WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합. Rabbit IgG 기반으로 항원 친화 크로마토그래피로 정제. 651/673 nm 형광 특성, 4°C 암소 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:10–1:1,000 |
| ELISA | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Pig, Rabbit, Rat, Dog, Chicken, Horse, Hamster, Human, Mustelid, Mouse, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Partial peptide sequence (between amino acids 1100–1300) of human ABCA1 gene. Actual sequence proprietary. |
| Conjugate | DyLight™ 650 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Liquid |
| Concentration | 0.77 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153645 |
Target Information
Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) are associated with Tangier disease (TD), an autosomal recessive disorder characterized by absence of plasma HDL and cholesterol ester deposition in the reticulo-endothelial system.
ABCA1 mediates apoA-I–associated export of cholesterol and phospholipids from cells. It is expressed on the plasma membrane and Golgi complex, regulated by cholesterol flux.
Regulation of cholesterol flux between HDL and macrophages involves competitive interaction between ABCA1 and SR-BI.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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