Thermo Fisher Scientific Phospho-Histone H2A.X (Ser139) Polyclonal Antibody
DNA 손상 반응 연구용 phospho-Histone H2A.X (Ser139) 폴리클로날 항체. 다양한 종에서 반응하며 Western blot, IHC, ICC 등 다중 응용 가능. 항원 친화 크로마토그래피로 정제된 고품질 항체로 DNA 손상 인식 및 세포 반응 분석에 적합.
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Thermo Fisher Scientific Phospho-Histone H2A.X (Ser139) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC-F) | - | 1 publication |
| Immunohistochemistry - Free Floating (IHC-Free) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Histone H2A.XS139ph (phospho Ser139). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.16 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736382 |
Product Specific Information
- Positive Control:
HCT116 (untreated), HCT116 (30 µM cisplatin for 24 hr), HCT116 (UVB 50mJ for 2 hr / 6 hr), NIH-3T3 (30 µM cisplatin for 24 hr), PC-12 (30 µM cisplatin for 24 hr) - Predicted Reactivity: Rhesus Monkey (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histone H2A.X (H2AX) is a member of the histone H2A family, one of four core histones forming the nucleosome core particle. In eukaryotes, DNA double-strand breaks (DSBs) trigger phosphorylation of serine 139 at the carboxy terminus of H2AX, resulting in γ-H2AX. This phosphorylation can be detected by Western blotting or immunofluorescence to assess DSB frequency.
Phosphatidylinositol 3-kinases are implicated in H2AX phosphorylation, though it remains unclear if ATM is the primary kinase or if DNA-PK and ATR also contribute. Structurally, H2A.X has 143 amino acids and is synthesized in both G1 and S phases. Its mRNA exists in two forms: one with a polyA tail (~1600 bases) and one without (~575 bases). The gene maps to 11q23.2–q23.3 on human chromosome 11.
Histone H2A.X plays a critical role in DNA structure and repair by regulating MDC1 binding at DNA damage sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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