Invitrogen™

Gateway™ pET-DEST42 Vector

The Champion™ pET-DEST42 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific자세히 알아보기

The Champion™ pET-DEST42 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific recombination and subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3) E. coli. The Champion™ pET-DEST42 destination vector offers:

• Bacteriophage T7_lac_ promoter for high-level expression
O operator sequences for lac repressor binding to ensure tighter regulation of transcription
• pBR322 ori for minimal basal expression
• C-terminal V5 and 6xHis tags for efficient detection and purification
R sites for efficient recombination with any _att_L-flanked Gateway™ entry vector

사양

구성 또는 유도성 시스템Inducible

유도제IPTG

프로모터T7, lacO

제품 유형Gateway System Destination Expression Vector

선택 제제(진핵)None

항생제 내성 박테리아Ampicillin (AmpR)

단백질 태그His Tag (6x), V5 Epitope Tag

클로닝 방법Gateway

수량6 μg

벡터pET, pDEST

제품라인Gateway™

Unit SizeEach