Thermo Fisher Scientific UACA/Nucling (Nuclear Membrane Marker) Monoclonal Antibody (AE-5)
UACA/Nucling 단백질을 표적하는 핵막 마커 단클론 항체로, Western blot, ICC/IF, Flow cytometry에 사용 가능. Human 및 Mouse 시료 반응성. 단백질 A/G 정제, 액상 형태, 4°C 보관. 세포 스트레스 유발성 세포사멸 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AE-5 |
| Immunogen | Nuclei of myeloid leukemia biopsy cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Regulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways: apoptosome up-regulation, LGALS3/galectin-3 down-regulation, and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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