Thermo Fisher Scientific ABCB11 Polyclonal Antibody
ABCB11 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 사용 가능. Human 반응성, Protein A 정제, PBS/glycerol 버퍼. 간세포 내 담즙 분비 관련 연구에 적합.
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Thermo Fisher Scientific ABCB11 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 474–745 of Human BSEP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.17 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545218 |
Product Specific Information
- Recommended positive control: Jurkat cells
- Predicted reactivity: Mouse (85%), Rat (87%), Dog (84%), Cat (87%), Rabbit (85%), Bovine (88%), Guinea pig (81%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ABCB11 is involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. It is expressed predominantly in the liver, localized to the canalicular microvilli and subcanalicular vesicles of hepatocytes. Structurally, ABCB11 is a multifunctional polypeptide with two homologous halves, each containing a hydrophobic membrane-anchoring domain and an ATP-binding cassette (ABC) domain.
Defects in ABCB11 cause progressive familial intrahepatic cholestasis 2 (PFIC2), an inherited liver disease of childhood characterized by cholestasis and normal serum gamma-glutamyltransferase activity.
Defects are also found in chronic intrahepatic cholestasis without a familial history of liver disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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