Thermo Fisher Scientific MIF Polyclonal Antibody
Human MIF 단백질을 인식하는 goat polyclonal antibody로 Western blot 및 IHC에 적합. 합성 펩타이드 기반 면역원 사용. 액상 형태이며 TBS buffer에 보관. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 0.01–0.03 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 5 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (NAANVGWNNSTFA) corresponding to the C-terminus amino acids of MIF (aa 103–115) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10981187 |
Product Specific Information
This antibody is predicted to react with mouse and rat based on sequence homology.
Tested in Peptide ELISA: antibody detection limit dilution 128,000.
Target Information
MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989).
Recently, MIF was rediscovered as a pituitary hormone that acts as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995).
MIF is released from macrophages and T cells in response to physiological concentrations of glucocorticoids.
The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998).
MIF plays a critical role in the host control of inflammation and immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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