Thermo Fisher Scientific TOPO TA, Dual TOP10 (Supply Center Packaging)

Invitrogen™ TOPO™ TA Cloning™ Dual Promoter Kit (Supply Center Packaging)

The TOPO™ TA Cloning™ Dual Promoter Kit enables fast and efficient cloning followed by in vitro transcription/translation.
This kit includes the pCR™II-TOPO™ TA vector, eliminating time-consuming restriction site cloning. TOPO™ cloning requires only 5 minutes using a simple 3-step protocol and yields up to 95% recombinants.

주요 특징

• 3′-T overhangs for direct ligation of Taq-amplified PCR products
• T7 and SP6 promoters for efficient in vitro transcription
• M13 forward and reverse primer sites for sequencing
• 16 convenient restriction sites (including EcoRI) for insert excision or subcloning
• Kanamycin and ampicillin resistance for selectable markers in E. coli
• Easy blue/white colony screening for recombinant selection
• Includes OneShot™ TOP10 Competent Cells for high cloning efficiency

Supply Center Packaging

This product is supplied in a special Supply Center packaging, which includes an outer box that holds both the TOPO™ vector box and the competent cells box, ensuring complete kit delivery for Supply Center customers.

제품 사양