Thermo Fisher Scientific Phospho-POLR2A (Ser2, Ser1616) Polyclonal Antibody

Applications

Product Specifications

Target Information

DNA-directed RNA polymerase II subunit RPB1 (POLR2A) is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using ribonucleoside triphosphates as substrates. POLR2A is the largest subunit and a catalytic component of RNA polymerase II, which synthesizes mRNA precursors and various functional non-coding RNAs. It forms the polymerase active center with the second largest subunit.

RNA polymerase II consists of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, clamp element, and jaws that interact with the DNA template. During transcription initiation, the single-stranded DNA template strand is positioned within the central active site cleft of Pol II. A bridging helix from RPB1 crosses the cleft near the catalytic site and promotes translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site.

Transcription elongation is influenced by phosphorylation of the C-terminal domain (CTD) of POLR2A, which serves as a platform for assembly of factors regulating transcription initiation, elongation, termination, and mRNA processing. Gene expression levels depend on the balance between methylation and acetylation of CTD lysines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.