Thermo Fisher Scientific beta Galactosidase Polyclonal Antibody, HRP
Thermo Fisher Scientific의 HRP 결합 베타 갈락토시다제 폴리클로날 항체로, WB, IHC, ELISA, IP에 적합합니다. E.coli 유래 항원을 기반으로 제작되었으며, 낮은 백그라운드와 높은 특이성을 제공합니다. 연구용으로 사용되며, 장기 보관 시 -20°C에서 보관 권장.
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- 200-4336xxxxx (2개 옵션)
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific beta Galactosidase Polyclonal Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:250 |
| ELISA | 1:2,000–1:5,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Beta Galactosidase (E.coli) |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid; dilute only prior to immediate use.
Beta Galactosidase antibody is an IgG fraction purified from monospecific antiserum by delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, and purified or partially purified Beta Galactosidase (E.coli).
Cross-reactivity with Beta Galactosidase from other species may occur but has not been specifically determined.
Very low background staining has been reported in various assays.
Target Information
Beta Galactosidase encodes a glycosyl hydrolase 35 family enzyme. Alternative splicing produces multiple transcript variants, one of which encodes a preproprotein processed to the mature lysosomal enzyme.
This enzyme hydrolyzes terminal beta-linked galactose residues from gangliosides and glycoconjugates.
Mutations in this gene are associated with GM1-gangliosidosis and Morquio B syndrome.
Diseases related to GLB1 include GM1-Gangliosidosis Type I and II.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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