Thermo Fisher Scientific Vimentin Polyclonal Antibody
Vimentin 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, 다양한 조직 및 세포 샘플에서 WB, IHC, ICC, Flow Cytometry, ELISA에 사용 가능. 고순도 Protein A 정제, 안정적 액상 형태, 다양한 종에 반응.
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Thermo Fisher Scientific Vimentin Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Human, Mouse, Pig, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human Vimentin, amino acids 371–466 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It plays a role in intracellular protein transport between the nucleus and plasma membrane. Vimentin, co-expressed with desmin during early development, is later replaced by tissue-specific IFPs. It is involved in steroid synthesis regulation through cholesterol transport within lipid droplets. Phosphorylation of vimentin leads to cytoskeletal rearrangement facilitating cholesterol movement to mitochondria, enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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