Thermo Fisher Scientific Replication Protein A2 (RPA2) Monoclonal Antibody (RPA2/4774)
Replication Protein A2(RPA2)를 인식하는 mouse monoclonal antibody로, Western blot, IHC 등 다양한 응용에 적합. Human 시료 반응성. 단백질 복제, 수선, 재조합 연구에 유용. 4°C 보관, 24개월 안정성 보장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
| Immunoelectrophoresis (IE) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RPA2/4774 |
| Immunogen | Recombinant human full-length Replication Protein A2 (RPA2) protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Stability: Antibody is stable for 24 months.
- Positive Control: MCF-7, HeLa, or HepG2 cells; Human tonsil (IHC).
- Cellular Localization: Nuclear
Specificity Comments:
Recognizes a protein of 34 kDa, identified as a subunit of Replication Protein A (RPA), also known as human single-stranded DNA binding protein (HSSB).
RPA from human cells is a stable heterotrimer of 70 kDa, 32–34 kDa, and 11–14 kDa subunits (RPA70, RPA32, and RPA14 respectively).
It is involved in DNA replication, repair, and recombination.
RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase α and δ.
RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.
Target Information
Required for DNA recombination, repair, and replication.
The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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