Thermo Fisher Scientific eIF2B5 Polyclonal Antibody
Rabbit polyclonal antibody against human eIF2B5, validated for WB, IHC, and IP. Affinity purified and supplied as liquid at 1 mg/mL. Recommended for research use in studying protein synthesis regulation. Store at 4°C, shipped on wet ice.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 5–15 µg/mg lysate |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 550 and 600 of human eukaryotic translation initiation factor 2B, subunit 5 epsilon |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Rabbit
Target Information
This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (eIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and other EIF2B subunits are associated with leukoencephalopathy with vanishing white matter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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