Thermo Fisher Scientific CDC123 Polyclonal Antibody
Rabbit polyclonal antibody recognizing human CDC123 protein. Validated for WB, IHC(P), and ICC/IF applications. High purity via antigen affinity chromatography. Supplied as liquid form with PBS/glycerol buffer, suitable for long-term storage at -20°C.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human CDC123. Recombinant protein control fragment (Product #RP-97111). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.7 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2639558 |
Product Specific Information
Immunogen sequence:
KEHVLHCQFS AWYPFFRGVT IKSVILPLPQ NVKDYLLDDG TLVVSGRDDP PTHSQPDSDD EAEEIQWSDD ENTATLTAPE FPEFATKVQ
Highest antigen sequence identity to orthologs:
- Mouse: 85%
- Rat: 88%
Target Information
The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid dehydrogenase complex. The BCKD complex consists of three catalytic components:
- A heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1)
- A homo-24-meric dihydrolipoyl transacylase
- A homodimeric dihydrolipoamide dehydrogenase
E1 is a thiamine pyrophosphate (TPP)-dependent enzyme, catalyzing the irreversible first step in branched-chain amino acid oxidation. The BCKDHB gene encodes the beta subunit of E1. The complex also includes two regulatory enzymes: a kinase and a phosphorylase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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