Thermo Fisher Scientific TRIM28 VHH-8His-Cys-tag Recombinant Alpaca Monoclonal Antibody (SAA1235)
TRIM28 단백질을 표적으로 하는 알파카 유래 재조합 단클론 항체입니다. Western blot, ELISA, Immunoprecipitation 등 다양한 면역분석에 적합합니다. CHO 세포에서 발현되며, Metal-chelate chromatography로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications
- Western Blot (WB): Assay-dependent
- ELISA: Assay-dependent
- Immunoprecipitation (IP): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Alpaca / VHH |
| Expression System | CHO cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SAA1235 |
| Immunogen | Recombinant Human TRIM28 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Metal-chelate chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
Product Specific Information
- Compatible with Direct ELISA
Target Information
The KRAB (Kruppel-associated box) domain is approximately 45 amino acids in length and acts as a transcriptional repression domain found in numerous transcription factors.
Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome. These proteins repress transcription through interactions with KAP-1 (KRAB-associated protein 1), an 835-amino-acid polypeptide containing a RING finger, B boxes, and a PHD finger.
KAP-1 forms complexes with KRAB-domain transcription factors to enhance repression efficiency and interacts directly with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1).
KAP-1 targets KRAZ1 to centromeric heterochromatin regions containing HP1α, thereby regulating transcriptional repression.
Mutations in KAP-1 that bind KRAB but not HP1 lead to random KRAZ1 distribution and strong transcriptional activation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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