Thermo Fisher Scientific RALBP1 Monoclonal Antibody (OTI11B2), TrueMAB
RALBP1 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, ICC/IF, Flow, IP 등 다양한 분석에 적합. HEK293T에서 생산된 full-length human RALBP1을 면역원으로 사용. 고순도 친화 크로마토그래피 정제, 동결건조 형태로 안정적 보관 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | 4 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI11B2 |
| Immunogen | Full-length human recombinant protein of human RALBP1 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
RALBP1 specifically activates hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. It mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX), serving as the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes.
RALBP1 catalyzes transport of glutathione conjugates and xenobiotics, contributing to multidrug resistance. It also functions as a scaffold protein assembling endocytotic complexes during interphase and interacts with CDC2 to regulate endocytosis. One of its substrates is EPN1/Epsin.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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