Thermo Fisher Scientific ARF1 Polyclonal Antibody
ARF1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA, IP, Dot blot 등 다양한 응용에 적합합니다. Human, Mouse, Rat 시료에 반응하며, Affinity chromatography로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Dot blot (DB) | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to unique amino acid sequence on Rat ARF1 protein. The peptide was covalently modified post-synthetically to achieve desired antigenicity. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. These small guanine nucleotide-binding proteins stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The ARF family includes 6 ARF proteins and 11 ARF-like proteins, all part of the RAS superfamily.
ARF proteins are categorized as:
- Class I: ARF1, ARF2, ARF3
- Class II: ARF4, ARF5
- Class III: ARF6
Members of each class share a common gene organization. The ARF1 protein localizes to the Golgi apparatus and plays a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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