Thermo Fisher Scientific NeutrAvidin Protein, DyLight 633

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:25,000

-

Immunohistochemistry (IHC)

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

1:200-1:1,000

-

ELISA (ELISA)

1:100 - 1:500

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Type

Protein

Conjugate

DyLight™ 633 DyLight™ 633 DyLight™ 633

Excitation/Emission Max

620/649 nm

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

22844 contains 1 mL of protein in blue color liquid at a concentration of 1 mg/mL. Fluorescence peak is 627 nm (deep red).

Target Information

Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia. The biotin-binding protein contains four identical subunits having a combined mass of 67,000-68,000 daltons. Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of 60,000 daltons. Carbohydrate-based lectin-binding is reduced to undetectable levels, yet biotin-binding affinity is retained. NeutrAvidin Protein offers the advantages of a neutral pI to minimize nonspecific adsorption, along with lysine residues that remain available for derivatization or other customized conjugation. NeutrAvidin Protein yields the lowest nonspecific binding among the known biotin binding proteins. The specific activity for biotin-binding is approximately 14 µg/mg of protein, which is near the theoretical maximum activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.