Thermo Fisher Scientific NeutrAvidin Protein, DyLight 633
DyLight 633 형광으로 표지된 NeutrAvidin 단백질로, 비오틴 결합 특이성이 높고 비특이적 결합이 최소화됨. WB, IHC, ICC, ELISA 등 다양한 면역실험에 적합. 620/649 nm 형광 특성으로 안정적인 신호 검출 제공.
- 카탈로그번호
- 22844
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
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Thermo Fisher Scientific NeutrAvidin Protein, DyLight 633
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 – 1:25,000 |
| Immunohistochemistry (IHC) | 1:200 – 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200 – 1:1,000 |
| ELISA | 1:100 – 1:500 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Type | Protein |
| Conjugate | DyLight™ 633 |
| Excitation/Emission Max | 620/649 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
Catalog No. 22844 contains 1 mL of blue-colored protein solution at a concentration of 1 mg/mL.
Fluorescence peak is 627 nm (deep red).
Target Information
Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles, and amphibians.
The biotin-binding protein contains four identical subunits with a combined mass of 67,000–68,000 daltons.
Removing glycosylation from avidin yields NeutrAvidin Protein with a mass of approximately 60,000 daltons.
This modification reduces carbohydrate-based lectin binding to undetectable levels while retaining biotin-binding affinity.
NeutrAvidin Protein offers:
- Neutral pI to minimize nonspecific adsorption
- Lysine residues available for derivatization or custom conjugation
- Lowest nonspecific binding among known biotin-binding proteins
The specific activity for biotin-binding is approximately 14 µg/mg of protein, near the theoretical maximum.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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