Thermo Fisher Scientific Goat anti-Chicken IgY (H+L) Secondary Antibody, DSB-X Biotin

Applications

Tested Dilution

Immunocytochemistry (ICC/IF)

1-10 µg/mL

Product Specifications

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

DSB-X Biotin DSB-X Biotin DSB-X Biotin

View additional formats

• [Alexa Fluor 488 ](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/Alexa Fluor 488/host/Goat/)
• [Alexa Fluor 546 ](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/Alexa Fluor 546/host/Goat/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/Alexa Fluor 555/host/Goat/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/Alexa Fluor 568/host/Goat/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/Alexa Fluor 594/host/Goat/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/Alexa Fluor 647/host/Goat/)
• [AP](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/AP/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/Biotin/host/Goat/)
• [FITC](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/FITC/host/Goat/)
• [HRP](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/HRP/host/Goat/)
• [Texas Red](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/Texas Red/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/TRITC/host/Goat/)
• Request custom conjugation

Form

Liquid

Concentration

2 mg/mL

Storage conditions

4° C, store in dark

RRID

AB_2536520

Target

IgY

Antibody Form

Whole Antibody

Product Specific Information

The DSB-X biotin goat anti-chicken IgG is prepared from antibodies that have not been cross-adsorbed. DSB-X biotin is a derivative of desthiobiotin, a stable biotin percursuor. DSB-X biotin utilizes a seven-atom spacer to increase the available of the DSB-Xbiotin conjugate to bind in the deep biotin-binding pocket of streptpavidin or avidin. Whereas harsh chaotropic agents and low pH are required to dissociate a biotin complex from avidin or steptavidin, binding can be reversed by adding excess D-desthiobiotin or natural D-biotin at neutral pH and at room temperature.

Target Information

Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for the chicken immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.