Thermo Fisher Scientific SQSTM1 Monoclonal Antibody (OTI5D5), TrueMAB
Human SQSTM1 단백질을 인식하는 mouse monoclonal antibody로, Western blot 및 IHC(P) 분석에 적합. TrueMAB 형식으로 높은 특이성과 재현성 제공. Lyophilized 형태이며 PBS buffer에 trehalose 포함. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5D5 |
| Immunogen | Full-length human recombinant SQSTM1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The SQSTM1 gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the NF-κB signaling pathway. It acts as a scaffolding/adaptor protein with TNF receptor-associated factor 6 to mediate NF-κB activation.
Alternative splicing produces multiple isoforms. Mutations in SQSTM1 are associated with sporadic and familial Paget disease of bone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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