Thermo Fisher Scientific Cullin 2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 40-310 of human Cullin 2 (NP_001185708.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cullin 2,uniProtId:Q13617-1,ncbiNodeId:9606,antigenRange:40-310,antigenLength:745,antigenImageFileName:PA5-120902_Cullin_2_Q13617-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120902_Cullin_2_Q13617-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.63 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914474

Product Specific Information

Positive test controls include: PC-3, K-562, HL-60, 293T, OVCAR3.

Immunogen sequence: LEYVERATWN DRFSDIYALC VAYPEPLGER LYTETKIFLE NHVRHLHKRV LESEEQVLVM YHRYWEEYSK GADYMDCLYR YLNTQFIKKN KLTEADLQYG YGGVDMNEPL MEIGELALDM WRKLMVEPLQ AILIRMLLRE IKNDRGGEDP NQKVIHGVIN SFVHVEQYKK KFPLKFYQEI FESPFLTETG EYYKQEASNL LQESNCSQYM EKVLGRLKDE EIRCRKYLHP SSYTKVIHEC QQRMVADHLQ FLHAECHNII RQEKKNDMAN M

Target Information

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.