Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), FITC, eBioscience
인간 HLA-A, B, C 항원을 인식하는 W6/32 클론의 FITC 결합 단일클론 항체로, Flow Cytometry 및 Immunocytochemistry에 최적화됨. 인간 및 영장류 반응성, 488 nm/520 nm 형광 특성, 세포면 MHC class I 분석에 적합.
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Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), FITC, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | 2 publications |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | 3 publications |
| Flow Cytometry (Flow) | 5 µL (1 µg)/test | 34 publications |
| Neutralization (Neu) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rhesus monkey |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | W6/32 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1633403 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- Biotin
- Brilliant UV 395 / 615 / 737
- Brilliant Violet 650 / 786
- Functional Grade
- NovaFluor Blue 510 / 610-70S / 660-120S / 690
- NovaFluor Red 710 / 725 / 755
- NovaFluor Yellow 590 / 660 / 755
- PE / PE-Cyanine5 / PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436 / 600 / 702
- Custom conjugation available
Product Specific Information
The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, and C. These antigens, associated with beta 2-microglobulin, are expressed on all human nucleated cells and play a central role in cell-mediated immune response and tumor surveillance. The W6/32 clone recognizes a non-polymorphic epitope shared among HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11–12 kDa chains. Cross-reactivity is observed in baboon, rhesus, and cynomolgus monkey.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells at 5 µL (1 µg) per test.
A test corresponds to staining a cell sample in a final volume of 100 µL (typically 10⁵–10⁸ cells/test).
Excitation: 488 nm; Emission: 520 nm; Laser: Blue.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with beta2-microglobulin. They present foreign determinants for recognition by cytotoxic T cells, playing key roles in antiviral and antitumor defense. Human HLA-class I antigens include HLA-A, HLA-B, and HLA-C molecules.
HLA and MHC antibodies are essential in Immunopeptidomics, aiding identification and characterization of neoantigens via high-performance liquid chromatography coupled to tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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