Thermo Fisher Scientific Synaptotagmin 7 Monoclonal Antibody (N275/14), FITC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

N275/14

Immunogen

Fusion protein amino acids 150-239 (Cytoplasmic C2A domain) of mouse Synaptotagmin-7 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Synaptotagmin 7,uniProtId:Q9R0N7-1,ncbiNodeId:10090,antigenRange:150-239,antigenLength:403,antigenImageFileName:MA5-45589_Synaptotagmin_7_Q9R0N7-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45589_Synaptotagmin_7_Q9R0N7-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

FITC FITC FITC

View additional formats

• Unconjugated
• APC
• PE
• PerCP
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine

Contains

no preservative

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932043

Product Specific Information

1 µg/mL of MA5-45589 was sufficient for detection of Synaptotagmin-7 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 45kDa. Does not cross-react with Synaptotagmin-6 (or others). Can identify other isoforms bands at approximately 65kD.

This antibody was formerly sold as clone S275-14.

Target Information

This gene is a member of the synaptotagmin gene family and encodes a protein similar to other family members that mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. A similar protein in rodents mediates hormone secretion and lysosome exocytosis. In humans, expression of this gene has been associated with prostate cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.