Thermo Fisher Scientific Synaptotagmin 7 Monoclonal Antibody (N275/14), FITC
Synaptotagmin 7 단백질을 검출하는 FITC 결합 마우스 모노클론 항체. Western blot, IHC, ICC/IF에 적합하며 인간, 생쥐, 랫트 반응성. 단백질 G 정제, 4°C 암소 보관. 연구용으로만 사용.
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Thermo Fisher Scientific Synaptotagmin 7 Monoclonal Antibody (N275/14), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N275/14 |
| Immunogen | Fusion protein amino acids 150–239 (Cytoplasmic C2A domain) of mouse Synaptotagmin-7 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932043 |
Available Formats
- Unconjugated (MA5-27654)
- APC (MA5-45588)
- PE (MA5-45591)
- PerCP (MA5-45590)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45589 was sufficient for detection of Synaptotagmin-7 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 45 kDa. Does not cross-react with Synaptotagmin-6 or others. Can identify other isoform bands at approximately 65 kDa.
This antibody was formerly sold as clone S275-14.
Target Information
Synaptotagmin 7 is a member of the synaptotagmin gene family and encodes a protein involved in calcium-dependent regulation of membrane trafficking in synaptic transmission. In rodents, it mediates hormone secretion and lysosome exocytosis. In humans, expression has been associated with prostate cancer. Multiple alternatively spliced transcript variants have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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