Thermo Fisher Scientific CENP-F/Mitosin Polyclonal Antibody
Human CENP-F 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, IP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공. FFPE 조직에서는 citrate buffer pH 6.0을 이용한 epitope retrieval 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 2350 and 2400 of human centromere protein F (Mitosin) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC on FFPE tissue sections, epitope retrieval with citrate buffer pH 6.0 is recommended
Target Information
This gene encodes a protein that associates with the centromere–kinetochore complex. The protein is part of the nuclear matrix during the G2 phase of interphase. In late G2, it associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and intracellular bridge in late anaphase and telophase, respectively, and is subsequently degraded. Its localization suggests a role in chromosome segregation during mitosis. The protein may form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft-versus-host disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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