
Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 350
DyLight 350으로 결합된 염소 유래 항-마우스 IgG(H+L) 2차 항체로, WB, IHC, ICC, Flow 등 다양한 면역실험에 적합합니다. 교차흡착 처리로 비특이적 반응을 최소화하였으며, 353/432 nm의 형광 특성을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:2,000 |
| Flow Cytometry (Flow) | 1:25–1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Conjugate | DyLight™ 350 |
| Excitation / Emission Max | 353 / 432 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C (protected from light) |
| Shipping Conditions | Ambient |
| RRID | AB_1965942 |
| Target | IgG |
| Cross Adsorption | Human, bovine, horse, rabbit, swine, goat, and rat serum proteins |
| Antibody Form | Whole Antibody |
Product Specific Information
Product #62273 has been successfully used in Western blot, IF, ICC, IHC, IP, and FACS applications.
It reacts with the heavy chains of mouse IgG and the light chains common to most mouse immunoglobulins, but not with non-immunoglobulin serum proteins.
Cross-adsorbed to minimize reactivity with human, bovine, horse, rabbit, swine, goat, and rat serum proteins.
Store protected from light at 4°C. For long-term storage, add an equal volume of glycerol (final 50%) and store at -20°C.
DyLight 350 Amax = 353 nm; Emax = 432 nm. Mole Dye/Mole Protein Ratio is lot-dependent.
Target Information
Anti-Mouse secondary antibodies are affinity-purified and specifically recognize mouse immunoglobulins.
They are useful for detection, sorting, or purification of target molecules and allow multiple detection systems (HRP, AP, fluorescence).
Secondary antibodies enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically generated by immunizing host animals with pooled immunoglobulins and further purified or modified to ensure high specificity.
Available Conjugate Formats
다양한 형광 및 효소 표지 형식으로 제공됩니다.
예: Agarose, Alexa Fluor 시리즈, DyLight 시리즈, FITC, HRP, PE, TRITC 등.
Custom conjugation 요청 가능.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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