Thermo Fisher Scientific CD11c Monoclonal Antibody (3.9), APC-eFluor 780, eBioscience
인간 CD11c를 인식하는 Mouse IgG1 단클론 항체로 APC-eFluor 780 형광체가 결합된 제품입니다. Flow cytometry용으로 최적화되어 있으며, 5 µL/test로 사용합니다. 633 nm Red laser로 여기되며, 780 nm에서 방출됩니다. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific CD11c Monoclonal Antibody (3.9), APC-eFluor 780, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.5 µg)/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor™ 780, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3.9 |
| Conjugate | APC-eFluor™ 780 |
| Excitation / Emission Max | 756 / 785 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573947 |
Additional Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- Biotin
- Brilliant UV 737
- Brilliant UV 805
- eFluor 450
- eFluor 506
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Product Specific Information
Description:
The 3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin alpha X chain. CD11c associates with beta2 integrin to form the CD11c/CD18 heterodimer, expressed on monocytes, granulocytes, macrophages, NK cells, dendritic cells, and subsets of T and B lymphocytes. The complex binds CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion.
Applications Reported:
Used for flow cytometric analysis.
Applications Tested:
Pre-titrated and tested on human peripheral blood cells. Recommended use: 5 µL (0.5 µg)/test in 100 µL final volume. Suitable for 10⁵–10⁸ cells/test.
Fluorophore Information:
APC-eFluor® 780 emits at 780 nm, excited by a 633 nm Red laser. Ensure instrument compatibility.
Handling Notes:
- Light-sensitive: protect vial and samples from light.
- Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark.
- Excitation: 633–647 nm; Emission: 780 nm; Laser: Red.
- Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD11, with CD18, forms a heterodimer adhesion molecule.
- CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils.
- CD11b: Expressed in monocytes, macrophages, NK cells, some B/T cells, granulocytes.
- CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and a subset of lymphocytes.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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