Thermo Fisher Scientific PGF Monoclonal Antibody (62526)
마우스 PGF 단백질을 인식하는 Rat IgG2a 단클론 항체. Western blot, ELISA, Neutralization, Inhibition assay에 적합. Protein G 정제 및 PBS+trehalose 용액 형태로 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| ELISA | 0.5–1 µg/mL |
| Neutralization (Neu) | 0.05–0.15 µg/mL |
| Inhibition Assays (IA) | 0.05–0.15 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 62526 |
| Immunogen | Recombinant, full length mouse protein, expressed in Sf21 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_795206 |
Product Specific Information
Neutralization: At a concentration of 0.05–0.15 µg/mL, this antibody blocks 50% of the binding of recombinant mouse PlGF2 (4 ng/mL) to immobilized recombinant human Flt1/Fc Chimera (4 µg/mL of a 100 µL solution coated per well) in an ELISA assay.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Placental growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family. PlGF is a pleiotropic factor that affects different cell types and regulates various biological responses. The activity of VEGF, one of the most potent angiogenic mediators, is partly modulated by PlGF. It plays a major role in vasculogenesis and angiogenesis in the human placenta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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