Applied Biosystems™

TaqMan™ Protein Assays Open Kit

The TaqMan™ Protein Assays Open Kit contains the TaqMan™ Protein Assays Oligo Probe Kit (4448549) and the TaqMan™ Protein Assays자세히 알아보기

The TaqMan™ Protein Assays Open Kit contains the TaqMan™ Protein Assays Oligo Probe Kit (4448549) and the TaqMan™ Protein Assays Buffer Kit (4448571) required for making TaqMan™ Protein Assay probes, along with the customer-supplied biotinylated antibodies. You supply the biotinylated antibodies and add them to streptavidin-oligonucleotides (prox-oligos) to make the 2 antibody-oligonucleotide assay probes utilized in binding. The resulting assay probes are utilized to help quantitate protein expression using gold standard TaqMan™ 5` nuclease chemistry with a workflow and sample quantity similar to our TaqMan™ Gene Expression and MicroRNA Assays. As the protein expression results are obtained on the same analytical platform, these revolutionary assays enable direct correlation of mRNA and⁄or miRNA expression to protein expression.

Key product features:
• Small Sample Size – use up to 500 times less sample input compared to traditional methods
• Sensitive Detection – directly measure in vitro protein-protein interactions with only a few thousand cells
• Revolutionary – directly correlate mRNA and⁄or microRNA to protein
• Simple Workflow – 1-step lysis sample preparation, universal protocol
• Quantitative – protein marker expression fold changes detected

Simple Workflow
You make TaqMan™ Assay probes by using your biotinylated antibodies. Then follow the same workflow used with our pre-designed assays: the sample prep step consists of a gentle, 1-step cell lysis using a buffered non-ionic detergent, with no further sample clean up or purification required. The crude lysate samples can be directly mixed with the paired assay probes from any Protein Assay Kit for the target binding step. The entire experimental process, from sample prep to data output, can be completed in about 5 hours (Figure 1).

For Research Use Only. Not for use in diagnostics procedures.

사양

검출 방법Primer-probe

GC-Rich PCR PerformanceLow

PCR 방법qPCR

반응 속도Fast

기술Proximity Ligation Assay

용도(장비)7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOnePlus™, Fast Mode

그린 기능Sustainable packaging

라벨 또는 염료FAM

패시브 참조 염료ROX (Pre-mixed)

제품라인TaqMan™

제품 유형Protein Assays Open Kit

수량4,000 reactions

타겟 특이도Not Target-Specific

핫 스타트No

반응 수4000 Reactions

샘플 종류Tissue, Cells

Unit SizeEach