Thermo Fisher Scientific Maxima SYBR Green/Fluorescein qPCR Master Mix (2X), 200 reactions
Ready-to-use 2X qPCR master mix optimized for SYBR Green/Fluorescein detection. Provides high specificity, sensitivity, and reproducibility for gene expression and pathogen detection. Compatible with Bio-Rad iCycler iQ and similar instruments. Includes...
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Thermo Fisher Scientific Maxima SYBR Green/Fluorescein qPCR Master Mix (2X), 200 reactions
Thermo Scientific™ Maxima SYBR Green/Fluorescein qPCR Master Mix (2X) is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. It contains Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer—only template and primers need to be added.
The SYBR Green I intercalating dye enables DNA detection and analysis without sequence-specific probes.
Maxima Hot Start Taq DNA Polymerase, combined with an optimized buffer, ensures PCR specificity and sensitivity. dUTP is included for optional carry-over contamination control using uracil DNA glycosylase (UDG). This mix enables reproducible, sensitive, and specific quantification of genomic, plasmid, viral, and cDNA templates. Compatible with most real-time thermal cyclers.
Highlights
- Specificity: Maxima Hot Start Taq DNA Polymerase and optimized buffer eliminate non-specific amplification and primer dimers
- Sensitivity: Detects low copy number targets
- Wide linear range: Accurate quantification across 9 orders of magnitude
- Reproducibility and convenience: Ready-to-use 2X master mix
Applications
- Gene expression
- siRNA validation
- Genotyping
- Pathogen detection
Includes
Maxima SYBR Green/Fluorescein qPCR Master Mix (2X) includes Maxima Hot Start Taq DNA Polymerase, dNTPs (including dUTP), and fluorescein passive reference dye in an optimized PCR buffer. Supplied with nuclease-free water.
Related Products
- Maxima SYBR Green/ROX qPCR Master Mix (2X)
- Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
- Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial
Specifications
| 항목 | 내용 |
|---|---|
| 검출 방법 | SYBR |
| 형식 | Tube |
| GC-Rich PCR Performance | High |
| PCR 방법 | qPCR |
| 중합효소 | Taq DNA Polymerase |
| 반응 속도 | Standard |
| 용도(장비) | BioRad iCycler iQ, BioRad MyiQ, BioRad iQ5 |
| 제품라인 | Maxima |
| 제품 유형 | Real Time PCR SYBR Master Mix |
| 수량 | 200 reactions |
| 충분 | 200 Reactions of 25 μL |
| 용도(애플리케이션) | Gene Expression |
| 농도 | 2X |
| 반응 수 | 200 reactions |
| 샘플 종류 | DNA, dsDNA |
| 부피 | 2 × 1.25 mL |
| Unit Size | Each |
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